Quantitative Separation of Radioactive Sterols and Bile Acids in Human Faeces.

نویسندگان

  • B LEWIS
  • N B MYANT
چکیده

A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination between the bile-acid and sterol fractions is between 0.7 and 3.8%. The method gives better results than one based on separation of the faecal steroids into saponifiable and unsaponifiable material.

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عنوان ژورنال:
  • Journal of clinical pathology

دوره 18  شماره 

صفحات  -

تاریخ انتشار 1965